科研团队
柏林研究员课题组
1. 个人简介：
柏林，博士生导师，北京大学基础医学院生物物理学系研究员，北京大学博雅青年学者。于2012年博士毕业于中科院生物物理研究所，2012至2016年在美国布鲁克海文国家实验室从事博士后研究，2016至2020年在美国Van Andel研究所担任高级研究科学家。主持国自然面上项目、海外青年高层次人才项目等。以第一或通讯作者（含共同）在Nature、Nat Struct Mol Biol、Nat Commun、Sci Adv等国际一流期刊发表研究论文二十余篇。
2. 研究方向与领域：
主要研究方向：病原微生物药物靶点发现与药物研发
实验室主要利用冷冻电镜等生物物理学与生物化学方法研究重要疾病相关蛋白质的结构与功能，重点关注真菌药物靶点的发现与基于结构的抗真菌药物研发。
3. 实验室成员：
研究生： 孙雷、游子龙、吕瑞卿、刘坤昭、王昭彬、王乐瑄、季萌
前排右起：王乐瑄、季萌、柏林（导师）、吕瑞卿；后排右起：游子龙、刘坤昭、王昭彬、孙雷
4. 主要代表作：
1) Zhao C#, You Z#, Chen D#, Hang J#*, Wang Z, Wang L, Zhao P, Qiao J*, Yun C*, Bai L*. Structure of a fungal 1,3-β-glucan synthase. Sci Adv. 2023 Sep 15;9(37):eadh7820. doi: 10.1126/sciadv.adh7820.
2) Chen D#, Wang Z#, Wang L, Zhao P, Yun C*, Bai L*. Structure, catalysis, chitin transport, and selective inhibition of chitin synthase. Nat Commun. 2023 Aug 8;14(1):4776. doi: 10.1038/s41467-023-40479-4.
3) Bai L* , Li H*. Structural insights into the membrane chaperones for multi-pass membrane protein biogenesis. Curr Opin Struct Biol. 2023 Apr;79:102563. (Review)
4) Zhao P#, Zhao C#, Chen D, Yun C, Li H*, Bai L*. Structure and activation mechanism of the hexameric plasma membrane H+-ATPase. Nat Commun. 2021 Nov 8;12(1):6439.
5) Bai L#*, Jain BK#, You Q#, Duan HD, Takar M, Graham TR*, Li H*. Structural basis of the P4B ATPase lipid flippase activity. Nat Commun. 2021 Oct 13;12(1):5963.
6) Bai L* , Li H*. Cryo-EM structures of the endoplasmic reticulum membrane complex. FEBS J . 2021 Feb 24. doi: 10.1111/febs.15786. (Review)
7) Bai L* , Li H*. Protein N-glycosylation and O-mannosylation are catalyzed by two evolutionarily related GT-C glycosyltransferases. Curr Opin Struct Biol. 2021 Jan 11;68:66-73. (Review)
8) Bai L*, You Q, Feng X, Kovach A, Li H*. Structure of the ER membrane complex, a transmembrane-domain insertase. Nature. 2020;584:475-478
9) Bai L#*, You Q#, Jain BK#, Duan HD, Kovach A, Graham TR, Li H*. Transport mechanism of P4 ATPase phosphatidylcholine flippases. Elife. 2020 Dec 15;9:e62163.
10) Bai L*, Kovach A, You Q, Hsu HC, Zhao G, Li H*. Atomic structure of the eukaryotic lipid flippase Drs2p-Cdc50p. Nat Commun. 2019; 10:4142
11) Bai L#, Kovach A, You Q, Kenny A, Li H. Structure of the eukaryotic protein O-mannosyltransferase Pmt1-Pmt2 complex. Nat Struct Mol Biol. 2019. 26:704-711
12) Bai L#, Li H. Cryo‐EM is uncovering the mechanism of eukaryotic protein N‐glycosylation. The FEBS journal. 2019; 286.9: 1638-1644.
13) Bai L#, Wang T, Zhao G, Kovach A, Li H. The atomic structure of a eukaryotic oligosaccharyl transferase complex. Nature . 2018; doi:10.1038/nature25755
14) Santos RLA#, Bai L#, Singh PK#, Murakami N#, Fan H, Zhan W, Zhu Y, Jiang X, Zhang K, Assker JP, Nathan CF, Li H, Azzi J, Lin G. Structure of human immunoproteasome with a reversible and noncompetitive inhibitor that selectively inhibits activated lymphocytes. Nat Commun. 2017. 8(1):1692.
15) Bai L#, Yuan, Z., Sun, J., Georgescu, R., O’Donnell, ME., Li, H. Architecture of the Saccharomyces cerevisiae Replisome. DNA Replication, 2017. 207-228. (Springer book chapter)
16) Noguchi Y#, Yuan Z#, Bai L#, Schneider S, Zhao G, Stillman B, Speck C, Li H. Cryo-EM structure of Mcm2-7 double hexamer on DNA suggests a lagging-strand DNA extrusion model. Proc Natl Acad Sci U S A. 2017. 114(45):E9529-E9538.
17) Bai L#, Jastrab JB, Isasa M, Hu K, Yu H, Gygi SP, Darwin KH, Li H. Structural Analysis of Mycobacterium tuberculosis Homologues of the Eukaryotic Proteasome Assembly Chaperone 2 (PAC2). J Bacteriol. 2017. 199(9). pii: e00846-16.
18) Yuan Z#, Riera A#, Bai L#, Sun J, Nandi S, Spanos C, Chen ZA, Barbon M, Rappsilber J, Stillman B, Speck C, Li H. Structural basis of Mcm2-7 replicative helicase loading by ORC-Cdc6 and Cdt1. Nat Struct Mol Biol. 2017. 24(3):316-324.
19) Georgescu R#, Yuan Z#, Bai L#, de Luna Almeida Santos R, Sun J, Zhang D, Yurieva O, Li H, O'Donnell ME. Structure of eukaryotic CMG helicase at a replication fork and implications to replisome architecture and origin initiation. Proc Natl Acad Sci U S A. 2017. 114(5):E697-E706.
20) Bai L#, Hu K#, Wang T#, Jastrab JB, Darwin KH, Li H. Structural analysis of the dodecameric proteasome activator PafE in Mycobacterium tuberculosis. Proc Natl Acad Sci U S A. 2016. 113(14):E1983-92.
21) Sun Q, Han C, Liu L, Wang Y, Deng H, Bai L*, Jiang T*. Crystal structure and functional implication of the RUN domain of human NESCA. Protein Cell. 2012. 3(8):609-17.
22) Tang L#, Bai L#, Wang WH, Jiang T. Crystal structure of the carnitine transporter and insights into the antiport mechanism. Nat Struct Mol Biol. 2010. 17(4):492-6.
5. 联系方式 :
柏林
北京大学基础医学院生物物理学系
E-mail: lbai@bjmu.edu.cn
电话：010-82805865
地址：海淀区学院路38号北京大学基础医学院邮编：100191